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High Performance Liquid Chromatography (HPLC) is also known as "High Pressure Liquid Chromatography", "High Speed Liquid Chromatography", "High Resolution Liquid Chromatography", "Modern Column Chromatography" and so on. High Performance Liquid Chromatography is an important branch of chromatography.

Subculture: After the primary cell culture is successful, it needs to be separated and cultured, otherwise the cells will have insufficient living space or excessive density, and nutritional disorders, which will affect cell growth. The process in which cells are separated and diluted from the original culture flask and transferred to a new culture flask is called subculture. Passage cells allow the expansion of cultured cells (to form cell strains), they can be cloned, and are easy to preserve, but may lose some special cell and differentiation characteristics. The greatest advantage of subcultured cells to form cell lines is to provide a large amount of long-lasting experimental materials to facilitate experiments. Equipment required for subculture experiment